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Stud Mycol 55(1): 65-74 2006
Copyright © 2006 CBS Fungal Biodiversity Centre
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Characterisation of Phomopsis spp. associated with die-back of rooibos (Aspalathus linearis) in South Africa

Johan C. Janse van Rensburg1, Sandra C. Lamprecht1,*, Johannes Z. Groenewald2, Lisa A. Castlebury3 and Pedro W. Crous2

1 ARC Plant Protection Research Institute, P. Bag X5017, Stellenbosch, 7599
2 Centraalbureau voor Schimmelcultures, Fungal Biodiversity Centre, P.O. Box 85167, NL 3508 AD, Utrecht, The Netherlands
3 USDA-ARS, Systematic Botany & Mycology Laboratory, Rm 304, Bldg 011A, Beltsville, MD 20705, U.S.A.

* Correspondence: Sandra Lamprecht, lamprechts{at}arc.agric.za

Die-back of rooibos (Aspalathus linearis) causes substantial losses in commercial Aspalathus plantations in South Africa. In the past, the disease has been attributed to Phomopsis phaseoli (teleomorph: Diaporthe phaseolorum). Isolates obtained from diseased plants, however, were highly variable with regard to morphology and pathogenicity. The aim of the present study was thus to identify the Phomopsis species associated with die-back of rooibos. Isolates were subjected to DNA sequence comparisons of the internal transcribed spacer region (ITS1, 5.8S, ITS2) and partial sequences of the translation elongation factor-1 alpha gene. Furthermore, isolates were also compared in glasshouse inoculation trials on 8-mo-old potted plants to evaluate their pathogenicity. Five species were identified, of which D. aspalathi (formerly identified as D. phaseolorum or D. phaseolorum var. meridionalis) proved to be the most virulent, followed by D. ambigua, Phomopsis theicola, one species of Libertella and Phomopsis, respectively, and a newly described species, P. cuppatea. A description is also provided for D. ambigua based on a newly designated epitype specimen.

Taxonomic novelties: Diaporthe aspalathi Janse van Rensburg, Castlebury & Crous stat. et nom. nov., Phomopsis cuppatea Janse van Rensburg, Lamprecht & Crous sp. nov.

Keywords Elongation factor 1-alpha gene / Diaporthe / endophytes / ITS / pathogenicity / Phomopsis die-back / systematics




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Stud Mycol, January 1, 2007; 57(1): 1 - 3.
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